Pfizer Senior Associate Scientist in Cambridge, Massachusetts

About Pfizer

A career at Pfizer offers opportunity, ownership and impact.All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have the opportunity to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the biopharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives.

Role Description

The Inflammation & Immunology Research Unit is seeking a highly motivated scientist to work in our expanding flow cytometry lab . The applicant should have a solid background in flow cytometry and cell sorting. The qualified candidate will be responsible for independently running FACS-based and other assays to support many early and late-stage projects. In addition, the candidate will help train others on flow cytometry concepts including planning experiments, proper use of equipment, and correct data analysis. The candidate should have a desire to build on their knowledge of flow cytometry and be excited to take on new technologies and methods pertaining to developing a state-of-the-art flow cytometry lab.

Responsibilities

QC instruments on a daily basis and troubleshoot problems; build machine configurations and change configurations as needed.Help train other users on all aspects of flow cytometry from planning experiments, acquisition and analysis.Perform cell sorting and manage sort requests.Characterize and qualify reagents to be used in assays, including FACS antibody panels and titers.Develop and perform multicolor staining assays for primary human and mouse phenotyping experiments.Work independently, seeking help as needed, to conduct various FACS-based screening assays for small molecule and protein therapeutics.Perform data analysis using FACSDiva and FlowJo applications.Interpret and summarize experimental data and report summaries in team meetings, as needed.Familiarity with emerging technologies such as CyTOF and desire to expand knowledge past traditional flow cytometry.Experience working with liquid handling devices, or cytometers as part of a robotic automation suite, desireable.

Qualifications

Successful candidates will have a BA or BS degree with 4 years flow cytometry experience in appropriate scientific area related to position.A degree in the following is strongly preferred: Cell biology, molecular biology, microbiology, immunology or biochemistry.Technical Skills:Have an understanding of flow cytometry fundamentals and experience with analyzers including: BD LSRFortessa, Miltenyi MACSQuant and Intellicyt iQue Screener PLUS. *Experience cell sorting on a BD FACSAria and Sony SH-800 machines. *Comfortable developing and running 6 color FACS panels.General ability working with Windows and MAC operating systems and computer hardware/devices.*Software proficiency: Microsoft Office, FACSDiva, FlowJo, MACSQuantify, Forecyte and Graphpad Prism.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Senior Associate Scientist Cambridge, Massachusetts 1053036-1852